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A small heat shock protein important in plant defense is targeted by the Xanthomonas axonopodis pv. manihotis HpaF Effector

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dc.contributor.advisorBernal Giraldo, Adriana Jimena
dc.contributor.advisorRestrepo Restrepo, Silvia
dc.contributor.authorGómez de la Cruz, Diana Camila
dc.contributor.juryZimmermann, Barbara Hanna
dc.contributor.juryGonzález Almario, Adriana
dc.date.accessioned2018-09-28T11:02:05Z
dc.date.available2018-09-28T11:02:05Z
dc.date.issued2017es_CO
dc.description.abstractXanthomonas axonopochs pv. manihotis (Xam) is the causal agent of bacterial blight in cassava (Manihot esculenta) and, like many plant pathogens, relies on the secretion of type three effectors (T3E) to disrupt plant defense and favor disease development. HpaF is a core T3E conserved among several Xam strains from diverse origins and has a role as suppressor of plant defenses elicited by Pattern Triggered Immunity (PTI). A cassava protein that interacts with HpaF was previously identified in our laboratory using a yeast-two-hybrid screen against a cassava cDNA library. This interactor was named HpaF Interactor 1 (HF11) and corresponds to an HSP20-like chaperone. This protein was thought to be important in A. thaliana plant defense, as a mutant line for the HF11 gene ortholog has PTI impairments. However, the same could not be concluded for the protein in cassava due to the complexity in the generation of mutants for this plant. Here we confirmed and validated the interaction between HpaF and HF11 using protein co-immunoprecipitation. We also showed that the HF11 mutant from A. thaliana is complemented when transformed with HF11 from cassava, confirming the functional homology of these proteins. This is the first approach towards the characterization of a cassava protein interacting with a Xam T3E.es_CO
dc.description.degreelevelMaestríaes_CO
dc.description.degreenameMagíster en Ciencias Biológicases_CO
dc.format.extent43 hojases_CO
dc.format.mimetypeapplication/pdfes_CO
dc.identifier.doi10.57784/1992/13919
dc.identifier.instnameinstname:Universidad de los Andesspa
dc.identifier.pdfu754030.pdf
dc.identifier.reponamereponame:Repositorio Institucional Sénecaspa
dc.identifier.repourlrepourl:https://repositorio.uniandes.edu.co/spa
dc.identifier.urihttps://hdl.handle.net/1992/13919
dc.language.isoenges_CO
dc.publisherUniandes
dc.publisher.departmentDepartamento de Ciencias Biológicasspa
dc.publisher.facultyFacultad de Cienciases_CO
dc.publisher.programMaestría en Ciencias Biológicases_CO
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAl consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.sourceinstname:Universidad de los Andeses_CO
dc.sourcereponame:Repositorio Institucional Sénecaes_CO
dc.subject.keywordXanthomonas axonopodis - Investigacioneses_CO
dc.subject.keywordYuca - Enfermedades y plagas - Investigacioneses_CO
dc.subject.keywordPatología vegetal - Investigacioneses_CO
dc.subject.themesBiología
dc.titleA small heat shock protein important in plant defense is targeted by the Xanthomonas axonopodis pv. manihotis HpaF Effectores_CO
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication
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person.identifier.cvlachttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000468800
person.identifier.gsidhttps://scholar.google.es/citations?user=vQ9yFZoAAAAJ
person.identifier.gsidhttps://scholar.google.es/citations?user=7_dVIeAAAAAJ
person.identifier.orcid0000-0002-3557-697X
person.identifier.orcid0000-0001-9016-1040
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