The present study, focused in studying aspects related to the reproductive biology of the glassfrog Centrolene solitaria, a species that lacks associated natural history information. Between March and October 2018, we studied a population of C. solitaria in a locality in the department of Huila, Colombia. We conducted nocturnal and diurnal surveys, we calculated mating rates and parental care for males, examined phenotypic correlates of mating success, and described some aspects of the natural history of the species. We found that the mating success of the males increased with the number of nights they remain active, but not with the body size or the calling-perch height. Parental care was carried out exclusively by males, and the period of attention to the eggs is extended. The egg positions, deposited in riparian vegetation, consist of an average of 15 eggs (12-23), located on the upper part of the leaves between 0.6 - 3.0 m in height. The advertisement call of C. solitaria consists of three to four notes pulsed at an average frequency of 6298.67 Hz (6201.3-6378.8 Hz); the mean note duration was 0.036 s (0.031-0.045 s), and the mean pulse duration was 0.017 s (0.011-0.032 s). The tadpole is fosorial, slightly pigmented, with an elliptical body from the lateral view, and an anteroventral mouth with a dental formula LTRF: 2 (2) / 3 (1).