Departamento de Ingeniería Biomédica

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https://hdl.handle.net/1992/52212
Encuentre en acceso abierto la producción académica, investigativa y de creación del Departamento de Ingeniería Biomédica de la Universidad de los Andes.

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Examinando Departamento de Ingeniería Biomédica por Autor "52699074"

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  • Miniatura
    PublicaciónAcceso abierto
    Caracterización in vivo de la bioimpedancia del cerebro del pez cebra adulto en los estadios de normoxia, hipoxia e hipoxia severa
    (Universidad de los Andes, 2016-01) Sánchez Lasso, Laura Marcela; Valderrama Manrique, Mario Andrés; Akle Álvarez, Verónica; 52699074; Arbeláez Escalante, Pablo Andrés; Cordovez Alvarez, Juan Manuel
    The adult zebrafish is being established as an ideal animal model for neurophysiology studies. As a therapy, neurostimulation is based on the chemical or electrical stimulation of the brain nuclei which can generate variations in the production of neurotransmitters. The electrical stimulus is given by parameters as the bioimpedance that determines the current reaching the tissue. By using the adult zebrafish as an animal model and innovative techniques, the object of the present study is to characterize the bioimpedance of the adult zebrafish brain in conditions of normoxia, hypoxia and severe hypoxia forward to fill an empty gap in the literature about adult zebrafish brain's bio-impedance and open the possibility for studying the action mechanism of the neurostimulation therapy in this animal model.
  • Miniatura
    PublicaciónAcceso abierto
    Exploring the role of the nlgn1 gene in the response to stress through a CRISPR Cas9-mediated nlgn1 zebrafish knockout
    (Universidad de los Andes, 2023-12-11) Ávila Matallana, Laura Catalina; Bloch Morel, Natasha Ivonne; Akle Álvarez, Verónica; 52863778; 52699074; Lattig Matiz, María Claudia; Muñoz Camargo, Carolina; Facultad de Medicina::Neurociencia Traslacional; Facultad de Ingeniería::Grupo de Ingenieria Biomédica
    Neuroligin 1 (Nlgn1) is a neuronal cell membrane protein that plays a critical role in synapse formation and remodeling processes. Previous studies have suggested that neuroligin 1 (nlgn1) mutations could contribute to the development of post-traumatic stress disorder (PTSD), a neuropsychiatric condition with an unclear genetic and molecular basis. Here, we aim to generate a nlgn1 zebrafish knockout line (KO) using CRISPR-Cas9genomeeditingandinvestigate the functional consequences of silencing nlgn1, in zebrafish. Knocking-out nlgn1 in one-cell embryos produces a strong neurological phenotype, that significantly decreases survival. We successfully crossed surviving KO individuals to generate an F1 and an F2 generation of the nlgn1 KO line. We performed behavioral test to evaluate the effect of silencing nlgn1 on stress behavior. We observed significant changes in the behavior of F1 generation individuals after the application of a chronic stress protocol. These results suggest thatnlgn1 has a role mediating stress response in zebrafish. By unraveling the role of nlgn1 in stress-related disorders, we aim to shed light on the molecular pathways involved in PTSD development and contribute to a better understanding of the underlying factors influencing the risk of developing PTSD.
  • Miniatura
    PublicaciónAcceso abierto
    Spatiotemporal changes in the expression of genes junb and nlgn3 associated with mate choice in the brain of the guppy (Poecilia reticulata)
    (Universidad de los Andes, 2022-06-28) Reyes, Angie Sofía; Bloch Morel, Natasha Ivonne; Akle Álvarez, Verónica; 52863778; 52699074; Cárdenas Parra, Luis Fernando; Cordovez Álvarez, Juan Manuel; Neurogenetica y comportamiento, IBIO
    Comprender la evolución de la elección de pareja requiere analizar los mecanismos y la evolución de las preferencias de apareamiento, en particular cómo los elementos genéticos difieren entre los contextos sociales y de apareamiento. Estudios transcriptómicos previos han brindado información importante sobre cómo se organizan las respuestas neurogenéticas asociadas con el comportamiento de apareamiento y han resaltado genes y vías genéticas asociadas con diferentes estímulos. Sin embargo, el papel específico de los genes identificados por estos estudios aún no está claro y no ha sido seguido por estudios funcionales más detallados. Aquí nuestro objetivo es estudiar los patrones de expresión espaciotemporal de dos genes elegidos en base a estudios transcriptómicos previos a través de la red de toma de decisiones sociales del cerebro (SDMN) en guppies hembras expuestas a estímulos sociales y de apareamiento. Analizamos la dinámica espacial de dos genes relacionados con la elección de pareja en el SDMN en dos puntos de tiempo diferentes, 10 min y 30 min después de la exposición al estímulo. Nuestro estudio tiene implicaciones importantes para comprender el comportamiento de elección de pareja y los mecanismos que controlan el comportamiento de apareamiento en el cerebro.
  • Miniatura
    PublicaciónAcceso abierto
    Spatiotemporal dynamics of brain expression of glutamatergic genes in response to social stimuli in Poecilia reticulata
    (Universidad de los Andes, 2022-06-23) Bittar Yepes, Amaury Fernando; Bloch Morel, Natasha Ivonne; Akle Álvarez, Verónica; 52863778; 52699074; Cordovez Alvarez, Juan Manuel; Reyes Barrios, Luis Humberto; Grupo de investigación en neurogenética y genómica del departamento de ingeniería biomédica
    Studying spatiotemporal patterns of gene expression in the brain is fundamental to understanding its evolution, function, and how behavior is generated. Recent work on the study of transcriptomic responses associated with mating dynamics in guppies of the species Poecilia reticulata has been highlighted for its implications in the framework of brain function. Thanks to this work, several gene families of interest in behavioral control have been identified, and it is now necessary to delve more specifically into each of these families. In this project, we focused on the glutamatergic pathway involved in regulating behavior, learning, and memory, particularly on the grin1 and gria3 genes. We studied the expression changes of these genes during mate evaluation, a crucial component of mating behavior. We evaluated the expression and localization of these genes by in situ hybridization (ISH) when a female observes an attractive male or another female. The results of ISH revealed the expression of both genes in most of the Social Decision-Making Network nuclei (SDMN). Also, we found an increase in the expression of gria3 when a female observes an attractive male for 10 and 30 minutes. On the other hand, no differences were observed in the expression of grin1. This work is the first to study the relevance of genes in the glutamatergic pathway during mating contexts in the SDMN.